Protein_Domain

Part:BBa_K5376002:Design

Designed by: JiaXin Gao   Group: iGEM24_TJUSX   (2024-10-01)


Core streptavidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 295
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the high expression of streptavidin/core streptavidin, which may lead to cytotoxicity, it is necessary to control its expression during the construction process to reduce the impact of cytotoxicity on plasmid construction.


Source

Please refer to BBa_K5376001 for the source of full-length streptavidin

References